Composite

Part:BBa_K5269022:Design

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


SpyTag-GH19Chitinase-His-Tag and BsmB I cut site(for GGA)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 885
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 20
    Illegal NgoMIV site found at 686
    Illegal AgeI site found at 641
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References